InflammaScreen™ Services
Rapidly screen for anti-inflammatory leads using Repligut® Planar
Inflammatory bowel diseases (IBD) are a major health concern worldwide
Inflammatory bowel diseases (IBD) are a major health concern worldwide, with an estimated 1.6 million cases in the US alone. While there are many treatments available, they are often ineffective and can cause harmful side effects.
A major reason successful IBD therapies remain elusive is that current model systems cannot replicate key mechanistic aspects of the epithelial inflammatory response in humans.
That’s why we developed InflammaScreen™ Services, a suite of pre-qualified, multiple readout assays using our flagship model RepliGut® Planar Transverse Colon. These assays are specifically designed to both speed up discovery and improve the human relevance of gastrointestinal inflammation-focused drug discovery.
Rapidly Screen for anti-inflammatory activities
Triage larger chemical sets to identify most promising candidates
Compare candidates to gain clarity on overlapping or discerning functionalities
Achieve fast iterative turnaround while optimizing function
Using InflammaScreen™ Services, you can:
1. Reliably evaluate the ability to augment or block intestinal epithelium response to proinflammatory cytokines using a pre-qualified assay with defined readouts.
assay schematic
Timeline
Fully mature RepliGut® Planar models are first pre-treated with test articles followed by treatment with TNFa. Cultures are pre-treated with your compounds. Pro-inflammatory cytokine TNF-a is added to initiate damage. TEER measurements are taken at 24- and 48-hours post-treatment; LDH and IL8 measurements are taken at 48 hours post-treatment.
2. Quickly receive results from three independent biological outputs
(cytotoxicity, barrier integrity, pathway-dependent cytokine release)
3. Reveal powerful mechanistic and functional insights
for better downstream decision making
Triage larger chemical sets to identify most promising candidates
Compare candidates to gain clarity on overlapping or discerning functionalities
Illuminate new pathways for innovation and discovery
Getting Started
Contact us to start your project and ship compounds to Altis
Test up to 10 compounds per plate for prevention of inflammatory damage and signaling.
Get results in as little as 3 weeks
Download the interactive platemap
Learn more about InflammaScreen™ services from our interactive platemap. Click the button below to download an excel spreadsheet.
Ready to Go With Your Gut?
Contact us to learn more about RepliGut® Model Kits and Services.
Get in touch with us
Have a question?
Want to learn more about RepliGut® Systems? Send us a Message
