A Stem Cell Derived Intestinal Model, Grown From Donor Tissue.
Altis is a stem cell company. We take intestinal stem cells straight from human donor tissue and rebuild the gut epithelial barrier in a dish, region by region.
We start with human stem cells, not a cell line
RepliGut® Systems is a stem cell derived intestinal model built from primary human tissue. We isolate intestinal stem cells directly from donor gut, in a region specific way, then expand them into a renewable biobank. Seeded onto a permeable membrane, those stem cells rebuild a polarized epithelial barrier, the same tissue that lines the human gut.
That single idea drives the whole platform. Because we start from real stem cells, the barrier we grow carries native human biology, and we can shape which cell types it contains. No immortalized tumor line, no animal surrogate, just human gut epithelium you can study directly.
How we build a stem cell derived intestinal model
The path runs from intact human tissue to a working barrier on your bench. We isolate the stem cells that sit at the base of the intestinal crypts, expand them, and plate them directly onto the transwell where they differentiate.
Human donor tissue
Stem cells sit at the base of every intestinal crypt.
Isolate crypt stem cells
We draw the stem cells out from the crypt base.
Expand into a biobank
Cells proliferate to fill many cryopreserved vials.
Seed and differentiate
Stem cells are seeded, then differentiate into functional epithelium.
The result is a human gut barrier you can dose, sample, and measure, ready in about a week. See it applied in RepliGut® Planar.
One platform, the native mix of gut cell types
Left to differentiate, our stem cells rebuild the epithelial barrier with the cell types found in the human gut. That means absorptive enterocytes, mucus producing goblet cells, and hormone secreting enteroendocrine cells, in physiologic ratios.
Because the platform is stem cell derived, we can also steer differentiation. By enriching one lineage, we build focused models for specific biology.
Tune the barrier toward the biology you need
The same stem cells that make a full barrier can be pushed toward one cell type. That is how two focused models come from the platform.
Enrich goblet cells: the mucus model
Drive goblet cell differentiation and the barrier builds a real mucus layer, for studying the gut mucus barrier and drug diffusion through it.
Explore the mucus modelEnrich enteroendocrine cells: the L-cell model
Enrich enteroendocrine L-cells and the barrier reports hormone signaling, for GLP-1 and gut hormone research and the gut brain axis.
Explore the L-cell modelModels built on the RepliGut® platform
Every RepliGut® model starts from the same stem cell derived intestinal barrier. Pick the format that fits your question.
RepliGut® Planar
The flagship 2D barrier, a polarized monolayer in a transwell with apical and basal access.
Explore PlanarRepliGut® Crypt
A crypt structured model that captures the proliferative stem cell zone of the gut.
Explore CryptRepliGut® Immune Co-Culture
The epithelial barrier with immune cells beneath it, to model epithelial and immune crosstalk.
Explore Co-CultureIntestinal Mucus
Goblet cell enriched to build a physiological mucus layer over the barrier.
Explore MucusL-Cell & Gut Hormone
Enteroendocrine enriched for GLP-1 signaling and gut hormone research.
Explore L-CellWhy the starting material matters
A model is only as human as the cells it comes from. Starting from primary human stem cells is what lets the barrier behave like real gut, and it is where regulators are steering preclinical science.
Real human biology
Primary donor stem cells, not an immortalized colon tumor line.
Region specific
Small intestine and colon regions, each isolated and biobanked apart.
Renewable supply
Expandable stem cells mean one donor can support studies for years.
Regulatory tailwind
Aligned with the FDA push toward human relevant New Approach Methodologies.
The FDA now actively advances New Approach Methodologies for preclinical testing.
Run it in your lab, or have us run it
RepliGut® Kits
Build the stem cell derived intestinal model on your own bench. Kits ship with the cells, plates, media, and support you need.
Explore kitsFull-service studies
Have our PhD level scientists design and run the study on human tissue, then deliver data ready for your model.
Explore servicesStart with real human stem cells
Tell us your region and your question. We will help you design the right RepliGut® study on a stem cell derived intestinal model.
Talk to our scientists