Human Intestinal Models That Capture the Complexity of the Human Gut.
RepliGut® is a human intestinal model grown from stem cells. It shows how a drug is absorbed, metabolized, and tolerated in human tissue, not in an animal or a decades-old cell line.
Most drugs fail in people. Legacy models are part of the problem.
A human intestinal model recreates the gut epithelium in the lab, so you can test drugs on real human biology before the clinic. Nine out of ten drugs that enter trials still fail, often because animal studies and cell lines like Caco-2 miss how the human gut actually behaves.
Altis changes the starting point. Our human intestinal models give scientists human-relevant data from day one, so fewer surprises appear later.
Human biology first. That is the whole point.
Three questions RepliGut answers better than Caco-2.
Absorption & metabolism
Predict how an oral drug is absorbed, transported, and metabolized in the human gut, using biology Caco-2 cannot reproduce.
In vitro DMPK and ADMEInflammatory disease
Model IBD and immune-epithelial crosstalk in human tissue, then test how therapies act where the disease lives.
Inflammatory bowel diseaseToxicity & safety
Screen for GI toxicity early, on human cells, so safety risks surface long before they reach a patient.
StemTox toxicity screeningOne human intestinal model, three formats.
We start with ethically sourced human intestinal stem cells and grow them into working gut tissue. Choose the format that fits your question.
RepliGut® Planar
A flat, reproducible monolayer for permeability, transport, metabolism, and toxicity, in a familiar Transwell format.
Explore Planar
RepliGut® Crypt
Native crypt architecture with stem, proliferating, and differentiated zones, for regeneration and repair biology.
Explore Crypt
Immune Co-Culture
Epithelium paired with immune cells to model inflammation and the crosstalk that drives intestinal disease.
Explore Co-CultureA new approach, and the rules are catching up.
RepliGut is a complex in vitro model, the kind of human-relevant test the FDA recognizes as a New Approach Methodology. These methods are designed to reduce reliance on animal studies.
The FDA NAMs program and the FDA Modernization Act 2.0 are opening the door to human models in preclinical research. Altis was built for exactly this moment.
Real human gut tissue, ready for the lab.
Source
Ethically sourced stem cells from real human donors, spanning duodenum, jejunum, ileum, and colon, held in our biobank.
Grow
Cells proliferate and differentiate into the cell types of the human gut epithelium, the same biology you need to predict.
Test
You get reproducible, physiologically relevant data for absorption, disease modeling, and toxicity screening.
Run it yourself, or hand it to our scientists.
RepliGut® Kits
Ready-to-use human intestinal models delivered to your bench, with the protocols and support to get clean data fast.
Explore kits
Custom Services
Our team designs and runs the study, from DMPK and permeability to InflammaScreen and StemTox, and delivers the data.
Explore servicesHuman intestinal models, in plain terms.
What is a human intestinal model?
A human intestinal model is lab-grown gut tissue that reproduces the human intestinal epithelium. RepliGut grows it from real human stem cells, so drug absorption, metabolism, and toxicity behave as they would in people.
How is RepliGut different from Caco-2?
Caco-2 is a single cancer cell line that models one donor and lacks normal gut cell diversity. RepliGut uses primary human stem cells from specific intestinal regions, giving more physiologically relevant permeability, metabolism, and toxicity data.
What can RepliGut measure?
Teams use RepliGut for oral drug absorption and DMPK, transporter and metabolism studies, IBD and inflammation modeling, and early GI toxicity screening, all on human tissue.
Are RepliGut models part of the NAMs the FDA supports?
Yes. RepliGut is a complex in vitro model, or CIVM, one of the New Approach Methodologies the FDA recognizes in nonclinical drug development. The FDA NAMs program and the FDA Modernization Act 2.0 support human-relevant models like these, evaluated for a defined context of use.
Ready to build on human biology?
Tell us what you are trying to predict. We will show you how RepliGut® human intestinal models fit your program, through kits or a custom study.
Get in touch with us
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