RepliGut is a unique, human stem cell-derived platform that recreates the intestinal epithelium in a continuous layer of stem and differentiated cells. Focus applications include: preclinical studies for compound screening, investigating the effects of diseases, and microbiome research.


RepliGut can be purchased as a product or Altis can provide services.

RepliGut Planar

The monolayer format produces a continuous layer of stem or differentiated cells on a flat and porous surface.

Each tissue sample on the RepliGut kit features a patent-pending biomimetic scaffold, which separates RepliGut cells from the cassette’s porous membrane and allows RepliGut cells to survive for a prolonged period of time. Without the RepliGut scaffold, stem cells cannot survive on the cassette.

Luminal and basal reservoirs allow compounds and additional cell types to interact with the epithelial cells for side specific assays.

RepliGut Planar was developed to replace Caco-2 cells (cancer cells) and animals, which are currently used as a part of compound screening during preclinical studies, before human testing. This 2D monolayer platform should provide much better results than current methods, since it uses proliferative stem cells and terminally differentiated cells.

RepliGut Kit

The form factor for RepliGut kit is a multiwell plate, which can be provided in 6, 12, 24, or 96 well sizes, depending on a scientist’s needs. Each well contains a cassette insert with an individual tissue sample.

RepliGut kits can be purchased with all the materials that scientists need to culture the cells in their own facility, including the transwell multiwell plate, stem cells and media.

RepliGut Planar Tissue Sample

RepliGut tissue constructs are polarized monolayers that express tight junction proteins, and can be tailored to be comprised of stem/progenitor cells, differentiated cells, or both. All major cell lineages in physiologic ratios can be achieved.

Multiple Donor Biobank

Allows customer to choose regional specificity of the small intestine and colon from donors of different demographic backgrounds.

More platforms currently under development

To purchase a RepliGut Kit, please contact us.